Improved Serum- and Feeder-Free Culture of Mouse Germline Stem Cells1
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چکیده
منابع مشابه
Improved serum- and feeder-free culture of mouse spermatogonial stem cells Running title: Serum-free culture of spermatogonia
Spermatogonial stem cells (SSCs) undergo self-renewal division, which can be recapitulated in vitro. Attempts to establish serum-free culture conditions for SSCs have met with limited success. Although we previously reported that SSCs can be cultured without serum on laminin-coated plates, the growth rate and SSC concentration were relatively low, which made it inefficient for culturing large n...
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Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation o...
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Mouse embryonic stem (mES) cells and mouse induced pluripotent stem (miPS) cells are commonly maintained on inactivated mouse embryonic fibroblast feeder cells in medium supplemented with fetal bovine serum or proprietary replacements. An undefined medium containing unknown quantities of reagents has limited the development of applications for pluripotent cells because of the relative lack of k...
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background: spermatogonial stem cells are subpopulation of spermatogonial cells in testis tissue that support beginning and maintenance of spermatogenesis. ubiquitin carboxy-terminal hydrolase l1 (uchl1) could be a specific marker for identification of spermatogonial stem cells including spermatogonial sperm cells (sscs) in testis tissue and during the culture; therefore we undertook this study...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2014
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod.114.122317